Development And Characterisation of Morinda Citrafolia Coupled Hydroxyapatite Nano Particle as an Anticariogenic and Remineralising Agent

Abstract

Aim

This study looked at how morinda citrifolia coupled hydroxyapatite nanoparticle was developed. It also checked its features as an agent to prevent tooth decay.

Materials and Methods

Five grams of M.citrifolia powder was made by grinding the noni fruit and drying it. Five grams of HAP (hydroxyapatite) were prepared using the wet chemical condensation method. This HAP was then finely ground. It was sent for SEM (scanning electron microscopy) and EDS (energy-dispersive X-ray spectroscopy) to detect HAP nanoparticles and check the elements. After HAP was ready, it was combined with Morinda citrifolia and sent for FTIR (Fourier-transform infrared spectroscopy) to find the functional groups. This helped determine the substances present. Finally, S.mutans and LB broth were prepared. They were then sent for PCR analysis and compared with a control group and the HAP+Morinda sample.

Results

FTIR results showed a strong bond between Morinda citrifolia and hydroxyapatite nanoparticles. EDX results provided their chemical makeup. SEM results showed their clear physical features. Real-time PCR analysis showed a decrease in the nucleic acid expression of Lactobacillus and S.mutans samples after treatment with the Morinda citrifolia-coupled hydroxyapatite nanoparticles.

Conclusion

This study shows a good bond between Morinda citrifolia and hydroxyapatite nanoparticles, seen with FTIR and EDX analyses. SEM results show their clear shape and size. Real-time PCR analysis shows a clear drop in the nucleic acid expression of Lactobacillus and S. mutans after treatment. This suggests they can kill germs. The Morinda citrifolia-coupled hydroxyapatite nanoparticles look promising for use in dentistry and medicine. More study is needed to understand how they work and if they can be used in clinics.