Vitamin A And E Quantification Using Spectrophotometric Methods

Abstract

            The study presents a detailed exploration of spectrophotometric methods for quantifying vitamins A and E. These vitamins play critical roles in human health, necessitating accurate and efficient analytical techniques for their measurement in various matrices like food products, pharmaceuticals, and biological samples. The research evaluates methods such as the Ferrozine-Fe(II) complex method, which leverages the antioxidant properties of vitamins A and E to facilitate metal ion reduction, and Raman spectroscopy, a more sophisticated approach used for precise analysis. Key findings indicate the Ferrozine-Fe(II) method is cost-effective, with sensitivity suitable for low concentrations of vitamins, while Raman spectroscopy provides advanced specificity and efficacy. The study highlights limitations like interference from other antioxidants and complex sample matrices, urging careful sample preparation. The work's contributions include optimization of reaction conditions, offering a valuable tool for routine analysis in clinical and industrial settings. The research underscores the potential of spectrophotometry as a reliable and economical alternative to advanced techniques like HPLC, promoting its broader application in resource-limited environments.